Using yeast – a talk by Chris White to the Scottish Craft Brewers
Chris White, founder of White Labs,
took time out from a conference to address a packed and attentive audience
at McCowans Brewhouse, Edinburgh on 20th September 2005. As his
talk in England had to be cancelled due to lack of interest, your scribe was
delighted to have the opportunity to meet him and indeed the Scottish Craft
Brewers in the UK’s brewing (if not real ale) capital.

Chris White studied Biochemistry at UC Davies where he took some classes at
the Brewing School out of interest. His interest deepened and he went to San
Diego to do postgraduate work on yeast in 1991. He then set up White Labs in
San Diego in 1995 in a small (90m²) facility which was outgrown by 1998 and
they moved to larger premises. During this period he was a keen home brewer
and while his interest has been maintained, the success of White Labs means
that he gets to brew rather less often.
Most of White
Labs’ production now goes to US microbreweries, typically producing 5000 -
10 000 bbl/pa. Note that corresponds to a brewlength of 50 bbl – contrast
with British micros where length is more like 5-30 bbl. (1 bbl = 1 brewer’s
barrel = 0.7 UK barrels = 1.2 hl).
Production is
very different to a dried yeast plant - typically fifty strains (out of 400
in the ‘fridge’ at ‑70 to ‑80C) will be produced each week. About 30% of
production is of ‘private’ strains. Most of production is lager yeast and
during the vintage 50% is wine yeast. However Chris’s heart is clearly in
his ales, as we’ll see later.

Microbiology
The history
of microbiology was, for the first few centuries, that of scientific
brewing. In 1680, Antonie van Leeuwenhoek, the pioneer of microscopy first
observed “little animalcules” in beer. The significance of these was not
fully appreciated until Pasteur (the last Frenchman to teach us anything
about brewing - Ed) demonstrated that wort would not ferment unless
inoculated with yeast around 1850. This was news! Brewers now realised that
cleanliness was key to sound beer production, at least those who stayed in
business did. Pasteur was without honour in his home country where small
breweries fell by the wayside conspicuously earlier than they did elsewhere.
Beer demands sterility
Chris stated
that the quality demands for brewing yeast are far higher than that for
bread - the destination of most yeast produced - as it is only used for one
cycle, as is distillers’ yeast. Even wine requires less purity as other
flavour elements disguise the characteristics of the yeast.
A
characteristic of brewing yeasts is that they do not produce phenolic off
flavours (POFs) and are thus known as POF-negative. Chris pointed out that
all wild yeasts are POF-positive and thus brewing strains have been created
by mutation and selection by brewers. Now those who have suffered phenols in
their beer might wonder who would want a POF-positive strain. Wine makers,
it would seem. The flavours in some wines at least overpower the POFs and
some wine flavours benefit from wild yeast “contamination”.
While brewers
started using single strains following Hansen’s development of single-cell
techniques at Carlsberg around 1880, they were first used for wine
production in the 1920s. (Wine producers are still strangely reticent about
their yeasts, allowing punters to romantically imagine that the yeast comes
from the grape skins - Ed.}
Anyway,
propagation has to be sterile. This is not a quick squirt of sodium
metabisulphite - this involves man-sized autoclaves and clean rooms. The
results aim to achieve less that 1 bug in 109 cells; Chris
reckons that the best dried yeast will achieve 1 in 107. For
comparison, your scribe seldom counts yeast in his brewery without spotting
something suspicious swimming about by the time he has counted 100 cells, so
there’s little reason for a home brewer to choose liquid yeast for sterility
alone - the benefits are in flavour.
Yeast performance
Chris listed
a range of reasons for yeast death, taken from Walker’s 1998 research at
Dundee. We select a couple of points from this. Firstly yeast can be killed
by shear forces when being pumped. However this should not be a problem for
those using magnetic stirrers at a modest swirl, which is sufficient to
aereate the wort and clear the evolved CO2. On CO2,
Chris reckons that a small amount of dissolved CO2 in a
fermentation will seriously reduce yeast performance. Even the small partial
pressure of CO2 built up under an airlock will have an effect. He
recommends racking or rousing to clear CO2 and introduce some
oxygen to ensure complete fermentation. Now this is similar the “dropping”
method practised by Brakspear’s and once advocated by Graham Wheeler.
However dropping is often advocated after about 36 hours of fermentation.
Chris is suggesting a bit later, but while there is still enough yeast
around to mop up the oxygen avoiding oxidation. He cited Sierra Nevada who
transfer at 1020, although acknowledges that few breweries do this.
Un-American activities?
Chris
compared the US and UK brewing traditions and described the US as having no
middle ground; beer tends to be either anonymous swill from the big boys or
aggressively hopped and strong from the micros. US brewers, he reckons are
not very interested in flavours created by yeast, sticking to four or five
‘boring’ strains. Indeed he noted that the traditionalists in this regard
were Anheuser-Busch and Coors who stick with their traditional strains which
have flavour and flocculation characteristics that would not recommend them
to new brewers.
He praised
the British for taking more of an interest in highly-flavoured, low strength
ales and making the most of yeast-derived flavours; White Labs use gas
chromatography analysis of head space for fusels and esters to characterise
their yeast. He advocates open fermenters for flavour and was greatly
relieved to discover that the Scottish Craft Brewers practice was open
fermentation. US brewers use sealed fermenters because they are much easier
to clean with cleaning in place (CIP) systems and small amounts of
aggressive chemicals
He felt that
closed fermentation practised in the US leads to greater concentration of
undesirable volatiles in the beer; hence US some craft brewers characterise
their Trappist yeast as “bubblegum” and prefer the Abbey strain. These two
yeasts will perform closer to type in open - or at least shallower -
fermentation systems. The ideal system he believes is open fermentation, in
a clean room. A quote: “I love open fermentation. I try to talk US
homebrewers into it. It gives different volatiles and fermentation is faster
because the yeast is healthier.”
Chris also
commented that while the Czech brewer Budweiser Budvar has clung to its
traditional yeast they did not appear to give any thought to the change in
its performance when they went from wooden fermenters to stainless cylindroconicals.
One seldom hears Americans saying how much better things were under the
communists and can only hope that Senator McCarthy does not read this
journal.
Using yeast
The audience
was surprised to hear that the yeast can remove a considerable amount of the
hop character from a beer if it overflows the fermenter or is skimmed; it
might be necessary to double hopping rates to account for this. The number
of times a yeast can be re-pitched depends on the cleanliness; Chris reckons
2-3 times at home and ten times commercially. Home brewers should be aware
of the contamination in domestic kitches and take appropriate care.
Top-cropped yeast is healthier. He states that yeast recovered from
bottle-conditioned beer is rarely very good due to stress – but “he would
say that, wouldn’t he”!
Chris reckons
that a single tube is sufficient to ferment 20 litres of ale wort, without a
starter. The brewers present generally preferred a starter, and he conceded
that there was nothing wrong with that, but stated that during the short
periods mentioned (12-24h) the main effect was to wake the yeast up – little
cell multiplication would occur. The White Labs tubes contain around
100 billion cells, equivalent to a ‘pint starter’. (This is about a quarter
of the amount in an 11-g pack of Nottingham Ale yeast. (The White Labs tube
gives a pitching rate of 5 million cells/ml into 20 litres. Recommended
commercial pitching rates for ale are 1 million per degree Plato (ie 4
brewers degrees). It’s up to the brewer whether to increase the amount of
yeast in a starter or in the fermenter – clearly many US brewers produce
prizewinning ales just pitcing straight – Ed). Chris suggests aereating the
wort for 30 minutes.
Temperature
control is key to a successful fermentation. The character of Belgian ales
in particular is determined by yeasts and fermentation temperature. To
retain esters (fruitiness), Chris suggests reducing the temperature of a
fermentation towards the end as the flavours are developed in the first 72
hours. It is also wise to remove the green beer to a closed fermenter
towards the end to reduce the risk of infection.
Lager
requires a lot more yeast – four vials might be required. He suggests a
starter or, as the substrates for ester formation are not present in the
first 12 hours of fermentation, the fermentation could be started at 21°C.
This allows yeast to multiply and the fermentation is then cooled to lager
temperatures – say 13°C. He concedes that this opinion is not universally
held. Similarly ales could be started at 21-24°C – even as high as 27 so
long as it is cooled within 12-24h.
One
final tip for those who have opened a vial and been sprayed by yeast. Crack
the top open as soon as the vial is removed from the fridge and continue to
release gas every hour or so until pitching.
Notes on specific strains
WLP002 has
very high flocculation and will in consequence pull out a lot of hop
character from the beer. The Edinburgh strain WLP028 in contrast is less
flocculent, preserving hop character.
The
high-gravity yeast WLP099 does not ferment maltotriose. In consequence to
get very high alcohol content it is necessary to use an enzyme. Home brewers
are reluctant to do this as the substance persists in the product
potentially leading to bottle bombs. With high-alcohol beers (around 20%)
this is not a problem as the alcohol will stop the yeast.
The German
lager yeast WLP830 is now their top seller (after WLP001 “Chico”) although
it is less popular in home brewing circles. It is exported to Japan, Brazil,
Taiwan – and indeed back to Germany.
And so, back
to the Waverley for the sleeper back to London. Haggis and neeps, Traquair
ale and Lagavulin in the lounge car provided a fitting end to a delightful
evening. Many thanks to Chris White for his time and openness, John
Braithwaite of the Hop & Grape (White Labs UK distributor) for coming north
and and providing samples and of course the Scots for organising the event.
Compiled by David
Edge from his notes and those of Les Howarth and Stuart McLuckie. Statements
marked “Ed” are his thoughts alone.